Pengaruh Level Ekstrak Daun Kelor Kering dalam Pengencer Air Kelapa Muda dan Kuning Telur terhadap Kualitas Spermatozoa Babi Landrace
DOI:
https://doi.org/10.59891/animacultura.v2i2.50Keywords:
coconut water, landrace pigs, dried moringa leaf extract, liquid semenAbstract
Purpose of this study was to determine the effect of dried moringa leaf extract (EDKK) in coconut water ( AKM ) and egg yolk ( KT ) diluent on the quality of semen landrace pigs. Semen was collected once a week using the massage method from 2 year old landrace male pig with normal reproductive organs. The diluted semen was stored in cold box at 18-200C and evaluated for motility, viability, abnormality and survival of spermatozoa every 8 hours. The design used was a completely randomized design consisting of six treatments and five replications so that there were 30 experimental units AKM 2.4 mL + KT 0.6 mL (P0), AKM 2.4 mL + KT 0.6 mL + EDKK 0.03 mL (P1), AKM 2.4 mL + KT 0.6 mL + EDKK 0.06 mL (P2), AKM 2.4 mL + KT 0.6 mL + EDKK 0.09 mL (P3), AKM 2.4 mL + KT 0.6 mL + EDKK 0.12mL (P4), AKM 2.4 mL + KT 0.6 mL + EDKK 0.15 mL (P5). The results showed that spermatozoa stored for 24 hours in 2.4 mL AKM diluent + 0.6 ml KT + 0.03 mL EDKK (P1) had 43% motility, 59.40% viability, 4.40 abnormality and survival 26.40 hours better (P<0.05 ) than the other five diluents, while the abnormalities and survival of spermatozoa in the five diluents were significantly different (P<0.05). The conclusion of this research is that the addition of 0.03 mL of dried moringa leaf extract to the egg yolk coconut water diluents is able to maintain the motility, viability, abnormalities survival of landrace pig spermatozoa for up to 24 hours of storage.
References
Abu, A. H., & Ahemen, T. (2013). Testicular Morphometry and Sperm Quality of Rabbit Bucks Fed Graded Levels of Moringa oleifera Leaf Meal (MOLM). ISSN: 1117-9996 Doi:10.4314/agrosh.v13i1.5
Ardana, B., & Putra, H. (2008). Manajemen Reproduksi, Produksi Dan Penyakit Ternak Babi. Denpasar: Udayana University Press. Bali. Doi:https://udayanapress.unud.ac.id/galleries/ternak-babi-manajemen-reproduksi-produksi-dan-penyakit
Arifiantini, R. I. (2012). Teknik koleksi dan evaluasi semen pada hewan. PT Penerbit IPB Press, Bogor. Doi: https://svmbs.ipb.ac.id/teknik-koleksi-dan-evaluasi-semen-pada-hewan/
Bergeron, A., Crête, M.-H., Brindle, Y., & Manjunath, P. (2004). Low-density lipoprotein fraction from hen’s egg yolk decreases the binding of the major proteins of bovine seminal plasma to sperm and prevents lipid efflux from the sperm membrane. Biology of Reproduction, 70(3), 708–717. ISSN 0006-3363 Doi: https://doi.org/10.1093/biolre/ioad179
Bonet, S., Briz, M., & Fradera, A. (1993). Ultrastructural abnormalities of boar spermatozoa. Theriogenology, 40(2), 383–396. ISSN:0093-691X Doi:https://doi.org/10.1016/0093-691X(93)90276-B
Fafo, M., Hine, T. M., & Nalley, W. M. (2016). Pengujian efektivitas ekstrak daun kelor dalam pengencer sitrat-kuning telur terhadap kualitas semen cair babi landrace. Jurnal Nukleus Peternakan, 3(2), 184–195. ISSN:2656-792X Doi: https://doi.org/10.35508/nukleus.v3i2.805
Garner, D. L. (2000). Spermatozoa and seminal plasma in reproduction in farm animals edited by ESE Hafez. Lippincott Wiliams and Wilkins. Philadelphia.
Gena, M. G. G., Foeh, N. D. F. K., & Gaina, C. D. (2021). EKSTRAK ETANOL DAUN KELOR (Moringa oleifera Lam.) SEBAGAI ANTIOKSIDAN DALAM PENGENCER SEMEN BABI LANDRACE BERBASIS AIR BUAH LONTAR. Jurnal Veteriner Nusantara, 4(1), 8. ISSN: 2540-7643 Doi: https://doi.org/10.35508/jvn.v4i1.6041
Hammerstedt, R. H. (1993). Maintenance of bioenergetic balance in sperm and prevention of lipid peroxidation: a review of the effect on design of storage preservation systems. Reproduction, Fertility and Development, 5(6), 675–690. ISSN:1448-5990 Doi:https://doi.org/10.1071/RD9930675
Kostaman, T., & Suatama, I. K. (2006). Studi Motilitas dan Daya Hidup Spermatozoa Kambing Boer Pada Pengencer Iris-Sitrat-Fruktosa= Motility and Viability Test of Boer Goat Spermatozoa at Tris-Citrat-Fruktosa Extenders. Jurnal Sain Veteriner, 24(1). ISSN:2407-3733 Doi:https://doi.org/10.22146/jsv.352
Kurniasih, E. (2013). Khasiat dan Manfaat Daun Kelor. Penerbit Pustaka Baru Press: Yogyakarta. Doi:https://onesearch.id/Record/IOS3605.INLIS000000000006436
Manu, A. E., Dato, T. D., Kapitan, Y., & Oematan, G. (2022). EFEK LAMA BIOKONVERSI OLEH JAMUR TIRAM PUTIH (Pleurotus ostreatus) TERHADAP KOMPONEN SERAT SABUT KELAPA TUA (The effect of bioconversion by the white oyster mushroom (Pleurotus ostreatus) to the fibrous component of old coconut fiber). JURNAL NUKLEUS PETERNAKAN, 9(1), 1–8. ISSN:2656-792X Doi:https://doi.org/10.35508/nukleus.v9i1.4234https://ejurnal.undana.ac.id/index.php/nukleus/article/view/4234
MataHine, T., & Burhanuddin, M. A. (2014). Efektivitas air buah lontar dalam mempertahankan motilitas, viabilitas dan daya tahan hidup spermatozoa sapi bali. Jurnal Veteriner, 15(2), 263–273. ISSN : 1411 - 8327 Doi: https://ojs.unud.ac.id/index.php/jvet/article/view/9719
Moussa, M., Martinet, V., Trimeche, A., Tainturier, D., & Anton, M. (2002). Low density lipoproteins extracted from hen egg yolk by an easy method: cryoprotective effect on frozen–thawed bull semen. Theriogenology, 57(6), 1695–1706. ISSN:0093-691X Doi:https://doi.org/10.1016/S0093-691X(02)00682-9
Shukla, S. N., Singh, B. B., Tomar, N. S., & Misra, B. S. (1992). Factors affecting spermatozoan motility in preserved semen. INDIAN VETERINARY JOURNAL, 69, 856. ISSN:0019-6479 Doi:https://www.cabidigitallibrary.org/doi/full/10.5555/19930107946
Situmorang, P., Triwulaningsih, E., Lubis, A., Caroline, W., & Sugiarti, T. (2000). Pengaruh Proline, Carnitine Terhadap Daya Hidup Spermatozoa Yang Disimpan Dalam Suhu 5oc (Chilling Semen). Jurnal Ilmu Ternak Dan Veteriner, 6(1), 1–6. Doi:http://download.garuda.kemendikbud.go.id
Toelihere, M. R. (1993). Inseminasi Buatan pada Ternak. Angkasa. Bandung.(2006). Pokok-pokok pikiran tentang perkembangan (bio) teknologi reproduksi di masa lalu, masa kini, dan masa yang akan datang dalam menunjang pembangunan peternakan di Indonesia. Fakultas Kedokteran Hewan I. Theriogenology, 38, 209–222. Doi:https://balaiyanpus.jogjaprov.go.id/opac/detail-opac?id=70290
Widjaya, N. (2011). Pengaruh Pemberian Susu Skim dengan Pengencer Tris Kuning Telur terhadap Daya Tahan Hidup Spermatozoa Sapi pada Suhu Penyimpanan 5oC. Sains Peternakan: Jurnal Penelitian Ilmu Peternakan, 9(2), 72–76. ISSN: 2548-9321 Doi: https://doi.org/10.20961/sainspet.v9i2.4796
Downloads
Published
How to Cite
Issue
Section
License
Copyright (c) 2024 Efraim Na'u, Petrus Kune, Frangky M. S. Telupere
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.
Most read articles by the same author(s)
- Simon Petrus Potret, Aloysius Marawali, Agustinus Ridolf Riwu, Petrus Kune, Kombinasi Sari Buah Semangka dalam Pengencer Kuning Telur dan Larutan Tris Terhadap Kualitas Spermatozoa Babi Landrace , Animal Agricultura: Vol. 2 No. 2 (2024): Animal Agricultura
- Kristina Loka, W. Marlene Nalley, Petrus Kune, Pengaruh Kombinasi Pengencer Susu Kacang Kedelai Sangrai dan Sitrat Modifikasi terhadap Kualitas Spermatozoa Babi Landrace , Animal Agricultura: Vol. 2 No. 1 (2024): Animal Agricultura
- Agustio Sarcyano Sola Woda, Petrus Kune, Aloysius Marawali, Pengaruh Level Minyak Kelapa Murni dalam Pengencer Air Kelapa Muda Terhadap Kualitas Spermatozoa Babi Landrace , Animal Agricultura: Vol. 2 No. 2 (2024): Animal Agricultura
- Daniel Padalani, Aloysius Marawali, Petrus Kune, Kirenius Uly, Pengaruh Penambahan Virgin Coconut Oil dan Vitamin C dalam Pengencer Sari Buah Semangka Kuning Telur Terhadap Kualitas Spermatozoa Babi Landrace , Animal Agricultura: Vol. 3 No. 1 (2025): Animal Agricultura
