Kombinasi Sari Buah Semangka dalam Pengencer Kuning Telur dan Larutan Tris Terhadap Kualitas Spermatozoa Babi Landrace

Authors

  • Simon Petrus Potret Faculty of Animal Husbandry, Marine and Fisheries, Nusa Cendana University
  • Aloysius Marawali Faculty of Animal Husbandry, Marine and Fisheries, Nusa Cendana University
  • Agustinus Ridolf Riwu Faculty of Animal Husbandry, Marine and Fisheries, Nusa Cendana University
  • Petrus Kune Faculty of Animal Husbandry, Marine and Fisheries, Nusa Cendana University

DOI:

https://doi.org/10.59891/animacultura.v2i2.79

Keywords:

egg yolk, pig semen, tris solution, watermelon juice

Abstract

in egg yolk diluent (EY) on the quality of spermatozoa in landrace pigs. In this study, the semen source was obtained from 1 male landrace pig aged 2 years in healthy condition, and had been trained in semen storage which was then diluted with treatment diluent, namely: T0: WMJ 80%+EY 20%, T1: WMJ 60% +EY 20%+TS 20%, T2: WMJ 40%+EY 20%+TS 40%, T3: WMJ 20%+EY 20%+TS 60%, T4: EY 20%+TS 80%. Diluted semen is stored at 15-20 ̊C and evaluated every 8 hours for motility, viability, abnormalities and survival. The results showed that the diluent WMJ 20%+EY 20%+TS 60% had higher quality (P<0.05) compared to the other four treatments, namely with motility (62.00±2.74%), viability (70.20±2.77%), abnormality (3.30±0.27%) and survival (32.00±0.00) hours. It was concluded that the diluent WMJ 20%+EY 20%+TS 60% could have a good influence in maintaining the quality of spermatozoa in landrace pigs.

References

Agarwal, Ashok, Sajal Gupta, and Rakesh Sharma. 2016. Eosin-Nigrosin Staining Procedure. Andrological Evaluation of Male Infertility: A Laboratory Guide, 73–77.

Arifiantini, R I, and B Purwantara. 2010. Motility and Viability of Friesian Holstein Spermatozoa in Three Different Extender Stored at 5oC. Journal of the Indonesian Tropical Animal Agriculture 35 (4): 222–226.

Arifiantini, Raden Iis, T Wresdiayati, and E F Retnani. 2006. Kaji Banding Morfometri Spermatozoa Sapi Bali (Bos Sondaicus) Menggunaican Pewarnaan Williams, Eosin, Eosin Nigrosin Dan Formol-Saline= Comparative Study of Bali Bull Cattle (Bos Sondaicus) Sperm Morphometry... Jurnal Sain Veteriner 24 (1): 222-226.

Ax, R L, M Dally, B A Didion, R W Lenz, C C Love, D D Varner, B Hafez, and M E Bellin. 2000. Semen Evaluation. Reproduction in Farm Animals, 363–3275.

Banaszewska, Dorota, and Katarzyna Andraszek. 2021. Assessment of the Morphometry of Heads of Normal Sperm and Sperm with the Dag Defect in the Semen of Duroc Boars. Journal of Veterinary Research 65 (2): 239–244.

Bebas, Wayan, Geovany Larastiyani Buyona, dan Made Kota Budiasa. 2016. Penambahan Vitamin E Pada Pengencer BTS® Terhadap Daya Hidup Dan Motilitas Spermatozoa Babi Landrace Pada Penyimpanan 15 C. Buletin Veteriner Udayana 8 (1): 1–7.

Campbell, John R, M Douglas Kenealy, and Karen L Campbell. 2009. Animal Sciences: The Biology, Care, and Production of Domestic Animals. Waveland Press.

Crespilho, A M, M F Sá Filho, J A Dell’Aqua Jr, M Nichi, G A Monteiro, B R Avanzi, A Martins, and Frederico Ozanam Papa. 2012. Comparison of in Vitro and in Vivo Fertilizing Potential of Bovine Semen Frozen in Egg Yolk or New Lecithin Based Extenders. Livestock Science 149 (1–2): 1–6.

Hine, TM., Burhanuddin dan Marawali, A. 2014. Efektivitas Suplementasi Filtrat Jambu Biji Dalam Pengencer Air Kelapa-Kuning Telur Terhadap Kualitas Semen Cair Sapi Bali. Jurnal Veteriner Maret 20 (1): 20–29.

Fila, W A, E H Itam, J T Johnson, M O Odey, E E Effiong, K Dasofunjo, and E E Ambo. 2013. Comparative Proximate Compositions of Watermelon Citrullus Lanatus, Squash Cucurbita Pepo’l and Rambutan Nephelium Lappaceum. International Journal of Science and Technology 2 (1): 81–88.

Foeh, Nancy Diana Frederika Katerina. 2015. Dengan Ini Saya Menyatakan Bahwa Tesis Berjudul Kualitas Semen Beku Babi Dalam Pengencer Bts Dan Miii Menggunakan Krioprotektan Dimethylacetamide Dan Gliserol Dengan Sodium Dedocyl Sulphate. Bogor Agricultural University (IPB).

Garner, D L, and E S E Hafez. 2000. Spermatozoa dan Seminal Plasma. Reproduction in Farm Animals, 96–109.

Hoesni, F. 1997. Pengaruh Kadar Kuning Telur Dalam Berbagai Pengencer Terhadap Kualitas Spermatozoa Domba Pasca Pembekuan. Program Pasca Sarjana. Universitas Padjajaran, Bandung.

Johnson, J T, J A Lennox, U P Ujong, M O Odey, W O Fila, P N Edem, and K Dasofunjo. 2013. Comparative Vitamins Content of Pulp, Seed and Rind of Fresh and Dried Watermelon (Citrullus Lanatus). International Journal of Science and Technology 2 (1): 99–103.

Johnson, L A, K F Weitze, P Fiser, and W M C Maxwell. 2000. Storage of Boar Semen. Animal Reproduction Science 62 (1–3): 143–72.

Kaka, Aleksander, W Marlene Nalley, and Petrus Kune. 2014. Persentase Nira Lontar (Borassus Flabellifer L) Dalam Pengencer Tris-Kuning Telur Terhadap Kualitas Semen Cair Kambing Peranakan Etawah Yang Disimpan Pada Suhu 3-5 C. Jurnal Nukleus Peternakan 1 (1): 21–27.

MataHine, Thomas, dan Marawali A Burhanuddin. 2014. Efektivitas Air Buah Lontar Dalam Mempertahankan Motilitas, Viabilitas Dan Daya Tahan Hidup Spermatozoa Sapi Bali. Jurnal Veteriner 15 (2): 263–273.

Nugroho, Wahyu Eko. 2003. Efektivitas Konsentrasi Kuning Telur Dan Plasma Semen Pada Bahan Pengencer Tris Terhadap Kualitas Semen Beku Kambing Saanen.

Pamungkas, F A. n.d. Anwar. 2013. Daya Tahan Hidup Spermatozoa Kambing Boer Dalam Pengencer Tris Kuning Telur Yang Disimpan Pada Temperatur Berbeda. Badan Penelitian Dan Pengembangan Pertanian 1 (12): 331–339.

Parera, F, Z Prihatiny, D F Souhoka, and M Rizal. 2009. Pemanfaatan Sari Wortel Sebagai Pengencer Alternatif Spermatozoa Epididimis Sapi Bali. J. Indon. Trop. Anim. Agric 34 (1): 50–56.

Partodiharjo, S. 1992. Fisiologi Reproduksi Hewan. Mutiara Sumber Widya. IPB. Bogor.

Riyadhi, Muhammad, and Muhammad Rizal. 2018. Viabilitas Spermatozoa Cauda Epididimis Kerbau Rawa Dalam Berbagai Konsentrasi Pengencer Air Kelapa Muda Dan Kuning Telur. Acta Veterinaria Indonesiana 6 (1): 38–43.

Salisbury, Glenn Wade, N L VanDemark, dan R Djanuar. 1985. Fisiologi Reproduksi Dan Inseminasi Buatan Pada Sapi.

Susilawati, Trinil. 2011. Spermatology. Universitas Brawijaya Press.

Widiastuti, Evie. 2001. Kualitas Semen Beku Sapi FH Dengan Penambahan Antioksidan Vitamin C Dan E. IPB (Bogor Agricultural University).

Widjaya, Nilawati. 2011. Pengaruh Pemberian Susu Skim Dengan Pengencer Tris Kuning Telur Terhadap Daya Tahan Hidup Spermatozoa Sapi Pada Suhu Penyimpanan 5oC.1 Sains Peternakan: Jurnal Penelitian Ilmu Peternakan 9 (2): 72–76.

Yulnawati, M A, dan Herdis Setiadi. 2005. Pemanfaatan Sari Buah Melon Dan Sari Wortel Sebagai Media Pengencer Alternatif Semen Cair Domba Garut. Protein 1 (2): 151–160.

Downloads

Published

31-10-2024

How to Cite

Potret, S. P., Marawali, A., Riwu, A. R., & Kune, P. (2024). Kombinasi Sari Buah Semangka dalam Pengencer Kuning Telur dan Larutan Tris Terhadap Kualitas Spermatozoa Babi Landrace. Animal Agricultura, 2(2), 593–601. https://doi.org/10.59891/animacultura.v2i2.79

Most read articles by the same author(s)